He worked as Associate Prof. & Head of the Dept. of Paediatric Surgery, Chittagong Medical College & Hospital, and in DMCH, Assoc. Prof. in Paediatric Surgery. Subsequently, he joined as Prof and Head of the Department of Paediatric Surgery in Mymensingh Medical College from 2010-2018. Prof Talukder was examiner of Postgraduate students and guided thesis a good number of postgraduate students under Dhaka University and BSMMU till date.
Currently, He is working as an Advisor, also Professor & Head of the Department of Pediatric Surgery at Japan East West Medical College Hospital.